Journal: BMC Molecular and Cell Biology

Article Title: Compression and tension variably alter Osteoprotegerin expression via miR-3198 in periodontal ligament cells

doi: 10.1186/s12860-019-0187-2

Figure Lengend Snippet: miR-3198 gain-of-function and loss-of-function experiments. Results of real-time RT-PCR analysis for miR-3198 ( a , b ), OPG and RANKL ( c , d ) expression in HPL cells after transfection of miR-3198 inhibitor ( a , c ) and miR-3198 mimic ( b , d ). n = 3. Biological triplicated. Fold change from the control is shown. Open bar indicates the fold change of OPG expression, and close bar indicates that of RANKL expression ( c , d ). The concentrations of OPG as measured by ELISA after transfection of miR-3198 inhibitor ( e ) and miR-3198 mimic ( f ) are shown ( n = 3). Results of real-time PCR analysis for miR-1207 ( g , h ), OPG and RANKL ( i , j ) expression in HPL cells under the transfection of miR-1207 inhibitor ( g , i ) and miR-1207 mimic ( h , j ). n = 3. Biological triplicated. Fold changes from the control are shown. * indicates P < 0.05 versus control, and NS indicating there was no significant difference between samples

Article Snippet: The concentration of OPG in the culture supernatant was measured using an OPG ELISA kit (Boster Biological Technology, Pleasanton, CA), according to the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Expressing, Transfection, Control, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

Journal: BMC Molecular and Cell Biology

Article Title: Compression and tension variably alter Osteoprotegerin expression via miR-3198 in periodontal ligament cells

doi: 10.1186/s12860-019-0187-2

Figure Lengend Snippet: miR-3198 regulates the mechanical stress-mediated change of OPG expression. Results of real-time RT-PCR analysis for OPG and RANKL expression in HPL cells in the compression ( a ) and tension ( b ) experiments. n = 3. Biological triplicated. Fold change from the control are shown. Cont, control; Inh, transfection of miR-3198 inhibitor; Mimic, transfection of miR-3198 mimic; press, compression; tens, tension; TF, transfection. Also shown are the OPG concentrations measured by ELISA in the compression ( c ) and tension ( d ) experiments ( n = 3). * indicates P < 0.05 versus control. † indicates P < 0.05 between samples. NS indicates there was no significant difference between samples. e and f Representative image of the western blotting for OPG was shown. g and h Relative band intensity of the western blotting for OPG. *: P < 0.05 versus control. †: P < 0.05 between the groups. NS, no significant difference between the samples

Article Snippet: The concentration of OPG in the culture supernatant was measured using an OPG ELISA kit (Boster Biological Technology, Pleasanton, CA), according to the manufacturer’s instructions.

Techniques: Expressing, Quantitative RT-PCR, Control, Transfection, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Heliyon

Article Title: Acteoside attenuates acute lung injury following administration of cobra venom factor to mice

doi: 10.1016/j.heliyon.2022.e11622

Figure Lengend Snippet: Acteoside reduces inflammatory mediators and adhesion molecules in serum and BALF of ALI mice. Before administration with CVF to induced ALI, mice were orally pretreated with different concentrations (100, 50, and 20 mg/kg/day) of acteoside or PDTC (100 mg/kg/day) or vehicle for 7 days. Serum and BALF were collected 1 h after CVF (35 μg/kg) i.v. injection for the ELISA detection. A–C: IL-6, TNF-α, and ICAM-1 levels in serum; D–F: IL-6, TNF-α, and ICAM-1 in BALF. Results were presented as mean ± SEM ( n = 8). # P < 0.05 and ## P < 0.01 were compared with the control group; ∗ P < 0.05 and ∗∗ P < 0.01 were compared with the CVF group. ALI, acute injury lung; CVF, cobra venom factor; PDTC, pyrrolidinedithiocarbamic acid; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α; ICAM-1, intracellular adhesion molecule; SEM, standard error of the mean.

Article Snippet: Cytokines in BALF and serum were determined with mouse ELISA kits (Boster Biological Technology Co., Ltd., Wuhan, China) for IL-6 (Cat. No. EK0411), TNF-α (Cat. No. EK0527), and ICAM-1 (Cat. No. EK0371).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Control, Combined Bisulfite Restriction Analysis Assay

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The effects of different concentrations of chemerin on ROS level, T-AOC and glucose uptake in the high glucose cell model. ( A ) The relative fluorescence intensity of ROS. ( B ) The detection of total antioxidant capacity. ( C ) The intake of glucose by cells. HG: high glucose; ROS: reactive oxygen species; T-AOC: total antioxidant capacity. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Fluorescence

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The effects of chemerin on the glucose tolerance, weight of fetal mice and placentas, fasting insulin levels, lipid metabolism and liver function. ( A ) The blood glucose of pregnant mice detected by IPGTT on D16.5 (* represents the comparison between the chow groups and the HFD groups; # represents the comparison between the HFD groups and the treated groups). ( B ) The body weight of fetal mice on D18.5. ( C ) The weight of mice placentas on D18.5. ( D ) The sizes of fetal mice and placentas on D18.5 after chemerin treatment. ( E ) The serum level of chemerin on D18.5. ( F ) The serum level of fasting insulin on D18.5. ( G ) The serum level of ALT on D18.5. ( H ) The serum level of AST on D18.5. ( I ) The serum level of HDL on D18.5. ( J ) The serum level of LDL on D18.5. ( K ) The serum level of TC on D18.5. ( L ) The serum level of TG on D18.5. HFD: high fat diet; FINS: fasting insulin; ALT: alanine aminotransferase; AST: aspartate transaminase; HDL: high-density lipoprotein; LDL: low-density lipoprotein; TC: total cholesterol; TG: triglyceride. Data were shown as mean ± SD ( n ≥ 3). # P < 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Comparison

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The localization and expressions of chemerin and DsbA-L in mice placenta of the HFD groups and the chow groups. ( A ) Staining of chemerin and DsbA-L by immunohistochemistry (scale bar = 100 μm). ( B ) Western blotting images of mice placentas. ( C ) Protein quantification of chemerin relative to β-actin. ( D ) AOD of chemerin in mice placenta. ( E ) AOD of DsbA-L in mice placenta. DsbA-L: disulfide-bond A oxidoreductase-like protein; HFD: high fat diet; LA: labyrinth; JZ: junctional zone; AOD: average optical density. Data were shown as mean ± SD ( n ≥ 3). ** P < 0.01, **** P < 0.0001

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Staining, Immunohistochemistry, Western Blot

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The roles and mechanisms of chemerin in the placental oxidative stress and insulin resistance in HFD pregnant mice. ( A ) Western blotting images of mice placentas of three groups. ( B ) Western blotting images of mice placentas between the chow groups and the HFD groups. ( C ) Western blotting images of mice placentas between the HFD groups and the treated groups. The HFD placenta samples presented in ( B ) and ( C ) were from the same several mice. ( D ) Protein quantification of AKT relative to β-actin. ( E ) Protein quantification of IRS1 relative to β-actin. ( F ) Protein quantification of STING relative to β-actin. ( G ) Protein quantification of DsbA-L relative to β-actin. ( H ) The activity of SOD in mice placenta. ( I ) The activity of GSH-Px in mice placenta. ( J ) The activity of CAT in mice placenta. ( K ) The content of MDA in mice placenta. HFD: high fat diet; AKT/PKB: protein kinase B; IRS1: insulin receptor substrate 1; STING: stimulator of interferon genes; DsbA-L: disulfide-bond A oxidoreductase-like protein; SOD: superoxide dismutase; GSH-Px: glutathione peroxidase; CAT: catalase; MDA: malondialdehyde. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques: Western Blot, Activity Assay

Journal: Molecular Medicine

Article Title: Chemerin alleviates the placental oxidative stress and improves fetal overgrowth of gestational diabetes mellitus mice induced by high fat diet

doi: 10.1186/s10020-024-01007-2

Figure Lengend Snippet: The long-term impacts of chemerin on blood glucose levels and body weight of maternal and fetal mice. ( A ) The postpartum blood glucose levels of the maternal mice at 8 weeks by IPGTT. ( B ) The blood glucose levels of the mice offspring at 8 weeks old by IPGTT. ( C ) The serum levels of chemerin in the maternal mice at 8 weeks after delivery. ( D ) The serum levels of chemerin in the mice offspring at 8 weeks old. ( E ) The changes of body weight of the mice offspring from 1 to 8 weeks of age. HFD: high fat diet. Data were shown as mean ± SD ( n ≥ 3). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Fasting insulin levels were measured using a mouse insulin ELISA kit (P01325, RayBiotech Life, USA) and the content of chemerin was assayed by the mouse chemerin ELISA kit (EK1330, Boster Biological Technology, China), assays were both according to the manufacturer’s protocols.

Techniques:

Journal: bioRxiv

Article Title: White adipose tissue undergoes pathological dysfunction in the TDP-43A315T mouse model of amyotrophic lateral sclerosis (ALS)

doi: 10.1101/2025.07.03.662925

Figure Lengend Snippet: (A) Concentration of leptin levels at diagnosis measured by ELISA in healthy controls and ALS subgrouped by sex. (B) Concentration of leptin levels at diagnosis in ALS and controls subgrouped by sex and disease progression: slow, normal and fast progression patients. (C) Concentration of leptin levels at diagnosis in ALS and controls subgrouped by sex and rate of progression in overweight patients. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Abbreviations: ALS, sporadic amyotrophic lateral sclerosis.

Article Snippet: Leptin plasma levels were measured using a Human Leptin ELISA Kit PicoKine® (Boster Biological Technology) with samples diluted 1:20 for males and 1:10 for females.

Techniques: Concentration Assay, Biomarker Discovery, Enzyme-linked Immunosorbent Assay